Science

How Fluorescent Dye Is Used In Flow Cytometry

Flow cytometry is the analysis of individual cells as they pass through a fluid stream. Components of the cells are labeled with one or more fluorescent dyes to identify the cells. A laser within the flow cytometer excites the fluorescent molecules to produce light at numerous wavelengths. The amount of fluorescent light that is emitted from the study is an indication of the type of cells that are within the sample. Flow cytometry is often used during cancer analysis, immunology and cell cycle analysis.

One of the primary steps required to perform cytometry analysis is the staining of single cells with fluorescent dyes. Labeling on the cells is based on whether the cells have existing fluorescent dye. Florescent dye labeling will be based on the availability of antibodies within the proteins. If the antibodies exist than the cells will be stained using antibodies. If the cells do not contain antibodies, than the cells will be stained with an unlabeled antibody, then a second staining will occur with the florescent labeled antibodies.

After the cells have been classified, flow cytometry analysis can be performed. The suspension cells will be loaded into a tube and placed into the flow cytometer. The cells will flow through the instrument and pass in front of the laser individually. As the cell is hit by the laser, light will scatter mainly in two directions. The light that scatters toward the front of the cell will indicate the cell size, while the side scatter will measure the quality of the elements within the cell.

The main purpose of the laser is to excite the florescent molecules within the cells. The light emitted from the processes is at a 525 nm. The photomultiplier tubes within the cytometer contain light filters that are able to identify the light produced by the florescent dye. The cytometer may have several photomultiplier tubes to identify the various wavelengths produced by the fluorescent molecules.

What Is A Flow Cytometer?

A flow cytometer is an integral technology that is used with protein engineering as well as many other biotechnology applications. Flow cytometers are usually fairly large machines that are used in biotechnology laboratories. The machines use a laser to study a particle’s chemical and physical structure. The laser needs to be of a single wavelength, it is then directed towards the particles that are suspended in a stream of liquid. Detectors in the machine are then pointed towards the location where the laser converges with the stream of liquid. After this is carried out, the light that reflects off of the many particles in the stream is then analyzed. Depending on the light’s brightness after it is reflected, the machine can then determine many physical and chemical features that the particles are comprise of.

The applications of flow cytometers are incredibly extensive, the machines are integral in many biological and biotechnological uses because they are such efficient machines. When using them, t

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